Two recent publications describe the use of DRAQ5 to aid the continuing search for new drugs to defeat infection by T. Cruzi.
DRAQ5 powerfully allowed identification of individual host cells by nuclear staining, secondary segmentation of their cytoplasm and identification and enumeration of the intracellular parasites by labelling kinetoplast DNA in a single fluorescence channel!
The methodology is compatible with high content screening (HCS) automation and automated image analysis for the identification of positive hits that inhibit parasitic infection.
References:
Alonso-Padilla, Julio, et al.
"Automated high-content assay for compounds selectively toxic to Trypanosoma cruzi in a myoblastic cell line."
PLoS Neglected Tropical Diseases 9.1 (2015): e0003493.
&
Silva, Fredson T., et al.
"Design, synthesis and antitrypanosomal activity of some nitrofurazone 1, 2, 4-triazolic bioisosteric analogues."
European Journal of Medicinal Chemistry (2016). Jun 1.
Accepted, in press.
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