Hypotonic vacuoles confirmed by DRAQ9

Fascinating new knowledge on hypotonic vacuoles from a collaborative effort between Kent State Univ, Hamburg and Salzburg.

Despite the well-described and long-understood rationale for vacuoles in lower order eukaryotes little work has been done to determine the conditions for and source of vacuoles in mammalian cell systems. This new work published in Scientific Reports and led by Prof. Michael Model (Kent State Univ.) further classifies watery vacuoles, their origins and includes a newly described vacuole resulting from hypotonic environments and specifically, it transpires, from a low-chloride environment.

To confirm that these indeed were lipid-encapsulated watery vacuoles the cells were labelled with lipophilic probes, including DRAQ9™.

Prof. Model commented in a recent product review: 
“..DiI that we tried before failed to stain the vacuoles, but with DRAQ9 the result was unequivocally positive: a bright staining was easily detectable under a confocal microscope..”

WHERE TO BUY DRAQ9

Reference:
Zook, E., Pan, Y.E., Wipplinger, A. et al. Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss. Sci Rep 14, 29354 (2024).

ASSCR 2023 - Spotlight on Cell Therapies

BioStatus products DRAQ5™ & DRAQ7™ are, of course, used in countless research articles in pursuit of novel cell therapies.  They are trusted by pharma, biotechs and leading research institutes globally.

All BioStatus products are manufactured to the highest standards, from synthesis to packaging, under ISO 9001:2015 certificated processes.

But why should YOU choose them for development, characterisation and quality assurance of cell therapies?

        Where to buy: Sapphire Bioscience - please visit their booth!

To help you, here are some key examples where they have been components of flow cytometric analysis and cell sorting relevant to cell therapies, with hyperlinks to detailed documents and citations:

• Phenotypic analysis: easily gate singlet nucleated cells from doublets and debris, &..
• Cell Cycle / DNA Content Analysis: no-lyse, no wash direct method with minimal sample prep

• Side population analysis: exclude dead cells from disturbing the data

• Monocyte purity check: in preparation of therapeutic macrophages 

• Determining very rare antigen-specific T-cells

• ISHAGE CD34+ Stem Cell Enumeration: more reliable gating 
• cell therapy QC review article
• poster from European ref. lab.

• DNA Double-Strand Break Detection: assessing DSB repair capacity post-cryopreservation (IFC) 

• Micronucleus Detection: high-throughput genotoxicity testing (IFC)

• Smarter Cell Health and Apoptosis Assays: allowing multi-parameter and temporal options

• Single Cell Analysis: industry-standard gating of cells or nuclei for transcriptomics & genomics

• Cell-killing capacity: temporal monitoring of cytotoxic function

• Phagocytosis function: track fluorescence from prey to predator cells

DRAQ7™ to the rescue..

Did you know? You can add DRAQ7™ to a pre-existing chromophore panel when it is later realised a viability dye is needed!  Or, you can use the same concept to create a virtual channel that simplifies any compensation challenges for the other components or even to simply extend the capacity of your cytometer!

See how here

Fluorescence microscopy and high-content imaging

DRAQ5™ has been cited in many thousands of articles as the nuclear counterstain for fixed and live end-point fluorescence microscopy and in high-content imaging screens for drug discovery. 

DRAQ7™ enables time-lapse monitoring of cell health, for many days without inherent toxicity yet faithfully reporting cell death events.

ASK US! If you have any questions or need to discuss a specific assay we are only too happy to help out. Send an email to: enquiry@biostatus.com

        Where to buy: Sapphire Bioscience - please visit their booth!

IFC = Imaging Flow Cytometry

#ASSCR2023

Autophagy UK Network Meeting 2022

BioStatus is delighted to once more support the Autophagy UK Network's annual meeting at the University of Warwick

Here are some dedicated resources for cell biology assays in the study of autophagy, mitophagy, ..

First, a few numbers: you can use BioStatus products with confidence, being cited in more than 12,000 peer-reviewed articles.  DRAQ5 and DRAQ7 have been cited in more than 1200 articles mentioning autophagy.

BioStatus's patented products are all UK inventions, manufactured in the UK, available direct for next-day delivery but also through carefully selected distribution partners.

Here are quick Google Scholar searches to help you.. 

Recent articles with autophagy in the title and using DRAQ5

Recent articles with autophagy in the title and using DRAQ7

DRAQ5 - the far-red, live-cell permeant DNA dye - enables multi-colour flow cytometry, fluorescence microscopy and high content imaging.  At its simplest it's a counterstain for nucleated object gating or identification while at its most sophisticated it's a live-cell reporter of cell cycle.  If you need cryo-EM then it is the perfect fiduciary marker that can bridge living tissue to ultrastructure (see blog).

DRAQ7 - the far-red, viability dye - allows you to add a viability dye into a flow cytometry panel where there doesn't seem to be room for one (see CYTO 2018 poster) or just enables smarter single tube apoptosis assays like AnnexinV / TMRM / DRAQ7 (see blog).  It's become a reagent of choice in time-lapse imaging of cell death / survival in 2D and 3D cultures, being non-toxic and non-interfering (see BLSW 2015 poster and as used in the lab of the opening keynote speaker Prof Sylvie Urbé [Liang, et al. 2015]).

What else can BioStatus offer your research?

Single cell genomics & transcriptomics..

Both DRAQ5 & DRAQ7 feature strongly in single-cell and single-nuclei sorting protocols to aid delivery of quality templates for amplification and sequencing (see blog).

Hypoxia & autophagy..

HypoxiTRAK enables the time-lapse reporting of the accumulated hypoxic experience of individual cells in both 2D and 3D cultures, in hypoxia chambers and by imaging or flow cytometry.  HypoxiTRAK's signal is consistent with Hif-1a expression.  HypoxiTRAK requires no antibody detection nor does it futile redox cycle unlike the nitroimadazole based chemistries. (see blog).

High resolution imaging..

If you need to immobilize cells, model organisms or microtissues to image these at high resolution for colocalizations, FRAP or organelle detail then CyGEL may provide the answer.  This thermo-reversible hydrogel is compatible with live cells and tissues and has ideal optical properties - clear, RI-matched to biological samples, negligible fluorescence.  

Live cell tracking..

DRAQ9 - a novel non-toxic, far-red cell probe to follow cell migration in time-lapse studies (see MMC 2019 poster)

Any questions?  Email us: enquiry@biostatus.com or visit www.biostatus.com

Nominated! DRAQfx shortlisted in industry awards

DRAQfx™ FIX & GO needs your vote for the Best New Life Sciences Product of 2017.

The winner will be announced at SelectScience's 20th anniversary Scientists' Choice Awards® at AACR '18.

If you don't already know, DRAQfx™ FIX & GO is the far-red nuclear counterstain that offers:

• tried and tested PERFORMANCE of DRAQ™ probes for fixed cell and tissue section immunofluorescence microscopy, cited in thousands of publications

• and the CONVENIENCE and simplicity of a benchtop dropper bottle

Thanks for voting for DRAQfx™ FIX & GO!

WHERE TO BUY

Scottish Society for Cytomics 3rd Annual Meeting 23/8/17 in Dundee

Conference announcement:
Scottish Society for Cytomics - 3rd Annual Meeting
Wednesday August 23rd, 2017 - Dundee University

BioStatus is a proud sponsor of this event:
We will exhibit, give a presentation and sponsor the 1st poster prize

Registration is free (even scot-free perhaps?): https://goo.gl/U9HLXE

#ScotSocCytom2017

Flow cytometry training courses - The Babraham Institute, UK

The Babraham Institute has announced new dates for its flow cytometry training courses. 

The course are modular, having been designed to build on knowledge from one module to the other and are therefore presented as two linked modules.  The remaining 2016 dates are:

Module 1:       
18th March
13th April
22nd September

Module 2:     
21st & 22nd April
3rd & 4th November

For more details of the courses please see:
http://www.babraham.ac.uk/science-services/flow-cytometry/flow-training

To sign up for them please email:
flow_training@babraham.ac.uk

Quoting the trainers:
"If you are new to flow cytometry or just want to learn more about this evolving technology, then these courses are ideal for you. We have now had over 100 people trained during these popular courses"

Flow Cytometry Events in Nov/Dec 2015

Some of the places for meeting other flow cytometrists in the coming months!

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Hear about DRAQ products to reduce sample preparation ..

Bi-annual Clinical Flow Cytometry Meeting 
Tuesday November 10th, 2015
Mercure Bristol Holland House Hotel
Bristol

Organisers: Ulrika Johansson and Ricardo Morilla
More info

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Get the latest on cell health in real-time with DRAQ7 ..

Advances in Cytometry
Friday November 13th, 2015
Biochemical Society
Charles Darwin House
Roger St., LONDON

Organised by RMS
More info

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London Cytometry Club Meeting
Thursday November 26th, 2015
Lincoln's Inn Fields, London

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Dutch Flow Cytometry Congress
(Nederlandse Vereniging voor Cytometrie)
Thursday 26th - Friday 27th November, 2015
Schouwburg Odeon, Zwolle
More info

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MACC Meeting (25th Anniversary Event!)
Thursday December 10th, 2015
Cambridge Research Inst., Addenbrooke's Campus

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M5 Universities Flow Group Meeting
Tuesday December 15th, 2015
Sutton Bonnington Campus, Univ of Nottingham
More info

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Oxford Biomedical Imaging Festival, St John's College October 20, 2015

BioStatus is delighted to be a sponsor of the Oxford Biomedical Imaging Festival

St John's College
Oxford
Tuesday October 20th, 2015

Talk to us about our novel solutions for high performance microscopy:
- Skip the queue for the UV microscope
- Image cell health in real-time and 3D
- Image whole live organisms, motile and non-adherent objects

More info

SBI2 Conference Boston September 15-17, 2015

BioStatus is delighted to be a sponsor and exhibitor at the 2nd annual conference of the Society for Biomolecular Imaging and Informatics (SBI2).

BioStatus products are widely used for high content screening methodologies:  DRAQ5 and CyTRAK Orange enable multi-colour assays with GFP-compatible nuclear counterstaining in live or fixed cells.  DRAQ7 uniquely allows real-time monitoring of cell health in 2-D or 3-D and better fits multi-colour apoptosis experiments.

2nd Annual SBI2 Conference
Harvard Medical School, Boston MA
September 15-17, 2015

Find full details of this forthcoming conference here:
http://www.sbi2.org/2nd-annual-conference/

Imperial College London - Flow Cytometry Course

BioStatus is delighted to be a sponsor of this annual training event.
Our products enable simplified procedures and multi-colour protocols.
Ask us about reducing your sample preparation or dead cell exclusion without using a single channel!

Imperial College London
South Kensington Campus
September 3-4, 2015

For full details of the training course visit:
http://www.imperial.ac.uk/life-sciences/research/facilities/flow-cytometry-facility/course/

MMC 2015, Manchester (June 29 - July 2)

BioStatus will exhibit at The Microscience Microscopy Congress (MMC) 2015

Find out how BioStatus products make better use of a core facilities' valuable resources and allow breakthrough applications in long-term, real-time assays, imaging of ex vivo micro-tissue environments and interfaces cell sorting and high-performance fluorescence microscopy. Find us at booth #95.

Exhibitor workshop: come along and hear about long-term cell health assays using DRAQ7. Workshop Room #4 on Wednesday July 1 at 13:15.

The congress is organised by the Royal Microscopical Society in association with the Electron Microscopy and Analysis Group of the Institute of Physics.

Venue: Manchester Central Convention Complex
Exhibition dates: June 30 - July 2, 2015

More info:
http://www.mmc2015.org.uk

CYTO 2015 Glasgow, UK (June 26-30)

30th ISAC congress goes local!
We're delighted to be an exhibitor - come and find us at booth #202
Find out what's new from BioStatus.

Venue: SECC, Glasgow
Exhibition dates: 27-30 June, 2015

More info:
http://cytoconference.org/2015/Home.aspx

Immunology 2015, New Orleans (May 8-12)

BioStatus will exhibit at Immunology 2015 in New Orleans.

Visit booth #552 to learn how BioStatus products can get you better results easier and faster in your immunology research:

- Simplify and reduce sample preparation of complex samples
- Exclude dead cells from your FACS analysis and sorting without wasting a channel!
- Image live non-adherent cells and tissues and get them back afterwards!
- Design better high content screens
- Explore hypoxia responses

Immunology 2015 - AAI Annual Meeting
Ernest N Morial Convention Center, New Orleans

May 8-12, 2015

More info: http://immunology2015.org

Immunology 2015 (May 8-12)

Come and visit our booth (#552) in the exhibition hall

Learn about no-lyse, no-wash analysis of complex samples like blood and BM
See how to exclude dead cells without using a channel on your cytometer!
Image cells and tissues live and recover them afterwards ..

IMMUNOLOGY 2015™
American Association of Immunologists Annual Meeting
Ernest N. Morial Convention Center, New Orleans, LA

May 8-12, 2015

Exhibition open: May 9-11 

ELRIG Research & Innovation '15 (Wed. March 18th)

ELRIG's successful Research & Innovation conference has moved to Trent Vineyard, Nottingham

Date: Wednesday 18th March, 2015

BioStatus is delighted to be an exhibitor!

We will also have a "snapshot" presentation in the 'Novel Methods for Toxicity Studies' session at 12.25 where we will focus on the value of DRAQ7 to cell health assays

To register: click ELRIG R&I      #ELRIGRI15

High Content Analysis 2015 (Jan 26-27)

CHI's High Content Analysis & Phenotypic Screening Meeting
January 26-27, 2015
San Diego Hilton Resort & Spa

BioStatus is delighted to be an exhibitor at this annual event.

Visit us to learn how our products can help your screening needs:
- 2D cell based assays
- 3D, spheroid, microtissue, organoid assays
- cell health, in vitro toxicity assays
- live organism immobilization
- channel-free dead cell exclusion in flow cytometry
- and more ..

Roy Edward will be available for discussions on your specific project, on the booth and in the sessions

For more info on the conference visit ..
www.highcontentanalysis.com

Laboratory Diagnosis of Haematological Malignancies

Laboratory Diagnosis of Haematological Malignancies

Wednesday 10th December 2014

The Hatton, London

BioStatus is delighted to support this focused meeting, organised by Ricardo Morilla and the Royal Microscopical Society.

To register please visit: 

http://www.rms.org.uk/event_laboratory diagnosis of haematological malignancies.shtml

or contact:

The Royal Microscopical Society, 37/38 St. Clements, Oxford, OX4 1AJ, UK,

Te: +44 (0) 1865 254760 Fax: +44 (0) 1865 791237

Email: Victoria Masters : victoria@rms.org.uk

Dutch Flow Cytometry Society (NVC) 19-20 Nov

Dutch Flow Cytometry Society - Annual Symposium

Zwolle, NL - Theater Odeon
November 19-20, 2014

BioStatus is delighted to be a silver sponsor of this event.

Take the opportunity to visit the BioStatus exhibit to find out how our products can improve and simplify your flow cytometry.

We will be presenting the latest data on DRAQ7 on the ability to exclude dead cells without even using a single channel!!

Pharmaceutical Flow Cytometry and Imaging 2014 (19-20 Nov)

Pharmaceutical Flow Cytometry and Imaging 2014

Venue: kindly hosted by GSK, Ware, UK
Date: November 19-20, 2014

Organised by ELRIG

This biennial meeting has become a firm venue in our calendar.  It's a great place to hear the latest technologies and their application to biopharmaceutical research and development.

BioStatus products perform cross-platform making them reagents of choice whether you are working in flow cytometry, fluorescence microscopy or high content screening applications.

You can meet with BioStatus personnel at this event to ask your burning questions!

Register for free, here:
http://elrig.org/portfolio/pharmaceutical-flow-cytometry-imaging-2014/

Advances in Flow Cytometry 2014 Meeting (6th November)

Advances in Flow Cytometry

When: Thursday November 6th, 2014
At: The Biochemical Society, Darwin House, 12 Roger Street, LONDON

Organised by the Royal Microscopical Society

www.rms.org.uk/advancescytometry2014



BioStatus is delighted to support this meeting.
BioStatus will present new data on DRAQ7 describing a novel application in flow cytometry and cell sorting.