Dr. Ina Laura Pieper & colleagues (Cytometry Part A (2016). DOI: 10.1002/cyto.a.22874) from Swansea University and Calon Cardio describe the combined use of CyTRAK Orange and DRAQ7 to robustly quantify leukocytes in whole blood samples.
CyTRAK Orange (Em max 610 nm) was used to gate the nucleated cells while DRAQ7 (Em max 694 nm) was used to quantify dead / damaged cells, alongside counting beads.
The methodology was specifically developed to monitor in human, bovine and ovine samples for in vitro testing of heart pumps (left ventricular assist devices, LVAD) where cell damage is a key concern, though it should be generally applicable.
Such testing is not possible by haematology analyser and requires the capabilities of a flow cytometer.
The combined use of CyTRAK Orange and DRAQ7 permits the additional use of violet-excited chromophores, FITC and APC for more detailed phenotypic analysis.
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