The earlier demonstrations of the capability of the imaging flow cytometer to provide an unattended, automated analysis for chemical genotoxicity or radiation exposure has now been supported by an inter-laboratory comparison study by scientists at GSK, Broad Institute and Swansea, Cardiff, Newcastle, and Cambridge Universities.
They demonstrated that the procedures on the Amnis Imagestream, which included DRAQ5 as a DNA counterstain, were robust across the different protocols and sites involved.
This now has the strong potential to change the approach to genotoxicity testing away from late stage development and to bring it further back in the drug discovery pipeline, to discover unsuitable structures earlier in the process.
Reference:
Wills, John W., et al. "Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning." Archives of Toxicology. Vol 95, pp 3101–3115 (2021)
Related BioStatus blogposts:
Automated Micronucleus Test (MNT)...
New DRAQ5 citations in drug discovery (see Imagestream/FlowSight section)
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