The powerful technique "CODEX" recently developed by the Stanford University laboratory of Garry Nolan enables multiplex imaging (and therefore deep phenotyping) of single biological samples of solid tissue, using iterative cycles of labeling compatible with a standard fluorescence microscope.
In two recent examples the inventing laboratory has utilised DRAQ5 as the nuclear counterstain, detected in the "Cy5" filter channel. DRAQ5 was applied in the final cycle at 50 µM (consistent with the concentration used in rapid histopathology analyses and on FFPE sections elsewhere).
DRAQ5's expected performance in this application opens up new panel design combinations and broadens microscope compatibility
References:
Goltsev, Yury, et al. "Deep profiling of mouse splenic architecture with CODEX multiplexed imaging." Cell 174.4 (2018): 968-981.
Phillips, Darci, et al. "Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma." medRxiv (2020). DOI: 10.1101/2020.12.06.20244913
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