A recent Leukemia paper by clinical scientists in Paris (Mathis, et al. demonstrates use of CyTRAK Orange to allow lysis-free analysis of whole bone marrows.
This CyTRAK Orange-dependent “live" or nucleated cell gate was combined with BV421, FITC, PC-7, APC and APC-AF700 coupled antibodies on a Beckman Coulter Navios. Forward & side scatter parameters are preserved in the presence of CyTRAK Orange.
Usefully, CyTRAK Orange is not excited by violet or red laser lines with no overlap with FITC allowing for multi-colour panels whilst avoiding time-consuming lysis procedures and resulting uncontrolled cell losses.
"..the aim of this study was to identify markers of dyserythropoiesis relevant for the diagnosis of MDS analyzed by selecting erythroblasts in a whole no-lysis bone marrow strategy by using a nuclear dye." The authors show statistical significance of key erythroblast markers CD36 and CD71 between MDS patient and control samples that they state should improve the classification of patients.
"..the aim of this study was to identify markers of dyserythropoiesis relevant for the diagnosis of MDS analyzed by selecting erythroblasts in a whole no-lysis bone marrow strategy by using a nuclear dye." The authors show statistical significance of key erythroblast markers CD36 and CD71 between MDS patient and control samples that they state should improve the classification of patients.
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Reference:
Reference:
Flow cytometric detection of dyserythropoiesis: a sensitive and powerful diagnostic tool for myelodysplastic syndromes
S Mathis, N Chapuis, C Debord, A Rouquette, I Radford-Weiss, S Park, F Dreyfus, C Lacombe, M C Béné, O Kosmider, M Fontenay and V Bardet.
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