The far-red, live-cell permeant DNA dye DRAQ5 has been combined with thrombocyte and lineage markers for analysis by Amnis Imagestream imaging flow cytometry to study megakaryocytes (MK) in bone marrow.
This approach improved quantitation of MK, further extended by polyploidy distributions and a direct indication of MK cell exhaustion, which appeared to be linked to the highest polyploidies.
Using DRAQ5 this was all possible without any cell fixation, avoiding unnecessary sample preparation steps!
See Niswander, et al. Cytometry Part A (2014) 85.4: 302-312.
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