Tuesday, 14 November 2017

New Guidelines for Cytometry and Sorting in Immunology

Download this open access 200-page article written by 231 co-authors, experts in their respective fields, aimed at all who use flow cytometry in their immunological research to be better equipped to achieve their experimental goals and be publication-ready.

The European Journal of Immunology (EJI) already insists upon the inclusion of gating strategies in submitted manuscripts and, therefore, it is highly appropriate that this 2-year long effort was headed by expert immunologists with editorial responsibilities at EJI and a strong commitment to the advancement of cytometric technologies.

These comprehensive guidelines include sections on equipment set-up, data acquisition & handling, barcoding, CyTOF, combinatorial & imaging cytometry, complete immune cell cytometric phenotypes, and much more.

Our far-red viability probe DRAQ7™ gets a prominent mention in the section on “Cell Death”, with particular reference to its ability to open up an additional “virtual” channel for the exclusion of dead cells in both analysis and sorting.

The issue can be found here:

and direct links to the guidelines: 


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