Download this open access 200-page article written by
231 co-authors, experts in their respective fields, aimed at all who use flow
cytometry in their immunological research to be better equipped to achieve
their experimental goals and be publication-ready.
The European Journal of Immunology
(EJI) already insists upon the inclusion of gating strategies in submitted manuscripts
and, therefore, it is highly appropriate that this 2-year long effort was
headed by expert immunologists with editorial responsibilities at EJI and a strong
commitment to the advancement of cytometric technologies.
These comprehensive guidelines
include sections on equipment set-up, data acquisition & handling,
barcoding, CyTOF, combinatorial & imaging cytometry, complete immune cell
cytometric phenotypes, and much more.
Our
far-red viability probe DRAQ7™ gets a prominent mention in the section on “Cell
Death”, with particular reference to its ability to open up an additional “virtual”
channel for the exclusion of dead cells in both analysis and sorting.
The issue can be found here:
and direct links to the
guidelines:
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