A recent paper explores the use of fluorogenic dyes to image the hypoxic response of cells via CYP450 enzymes. This is the first paper citing the use of the far-red fluorescent reporter HypoxiTRAK™ (BioStatus Limited), published by research groups at the Departments of Chemistry and Oncology, University of Oxford.
The authors describe the development and performance of bioreductive azide dyes. HypoxiTRAK™ is BioStatus's commercially available probe for the detection of a functional reductive potential or response in cells experiencing hypoxic stress, and used here to validate the performance of the authors' experimental molecules.
References:
O’Connor, Liam J., et al. "CYP450 enzymes effect oxygen-dependent reduction of azide-based fluorogenic dyes." ACS central science 3.1 (2017): 20-30. (Fig. S2).
UPDATE: HypoxiTRAK™ is also compatible with real-time/time-lapse monitoring of the hypoxic experience of cells in both 2D and 3D microtissues and spheroids, over several days. The latest paper from the expert lab of Prof. Paul A. Johnston, UPMC (Pittsburgh) can be found here:
Close, David A., and Paul A. Johnston. "Detection and impact of hypoxic regions in multicellular tumor spheroid cultures formed by head and neck squamous cell carcinoma cells lines." SLAS Discovery 27.1 (2022): 39-54.
Related products:
DRAQ9™ has been demonstrated for the tracking of cell migration and the monitoring of spheroid growth and integrity:
Poster presentation, SLAS 2020
DRAQ7™ has been widely used in 3D tumour microtissues to track cell death over time. Read recent blog reports:
Cancer Drug Screening in PDTOs - key parameters
Real-Time Cell Health Monitoring in 2D/3D with DRAQ7
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