Scientists at RIKEN, Kobe have successfully transplanted MHC-matched iPSC-derived retinal pigment epithelial cells (RPEs) without rejection.
The far-red cell permeant DNA probe DRAQ5 was deployed in an assay to demonstrate the phagocytotic function of the cells to take up FITC-tagged photoreceptor rod outer segment (ROS). From the DRAQ5 labelling the authors inferred that the cells under analysis were live/intact.
DRAQ5 rapidly and efficiently labels intact nucleated cells with stoichiometry. DRAQ5 is spectrally separated from FITC. It enables simplified analysis of complex samples that may contain damaged cells and debris while limiting the requirement for sample preparation.
The research in this work points to the possibility of successful transplantation of cells from MHC homozygous donors might be used to treat retinal diseases in histocompatible patients.
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