Focus on gamma-H2AX foci - 2 new papers

Two papers published in the the last week demonstrate the value of DRAQ5 and the ImagestreamX imaging flow cytometer:

Parris et al. (Brunel University, UK) describe an enhanced methodology to study DNA damage foci. Increased resolution of the Amnis ImagestreamX (Merck Millipore/EMD Millipore) imaging flow cytometer enables better confidence in the overlaying of  ɣ-H2AX foci spot count on top of the robust nuclear segmentation provided by far-red DRAQ5.

Parris, C. N., Adam Zahir, S., Al-Ali, H., Bourton, E. C., Plowman, C. and Plowman, P. N. (2015), Enhanced γ-H2AX DNA damage foci detection using multimagnification and extended depth of field in imaging flow cytometry. Cytometry, 87: 717–723. doi: 10.1002/cyto.a.22697

Link here:

http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22697/abstract

Endres et al. (SUNY, USA) have performed a detailed study of the impact of ROS on transcriptional and translational  pathways using a specific gene knockout.  One of the parameters of study was the quantification of ɣ-H2AX foci spots overlaid on the nuclear segmentation afforded by the far-red DNA probe DRAQ5 and imaged and analysed on the ImagestreamX.

Endres L, Begley U, Clark R, Gu C, Dziergowska A, Małkiewicz A, et al. (2015) Alkbh8 Regulates Selenocysteine-Protein Expression to Protect against Reactive Oxygen Species Damage. PLoS ONE 10(7): e0131335. doi:10.1371/journal.pone.0131335

Link here:

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0131335

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