DRAQ probes on MACSQuant cytometers

Here are a few examples where DRAQ5 and DRAQ7 have been used on Miltenyi Biotec's MACSQuant flow cytometers:

DRAQ7 - for dead cell exclusion

McKimmie et al., Blood 2013

http://dx.doi.org/10.1182/blood-2012-04-425314

Chemokine uptake assay into CHO cells: Alexa-647 labeled CCL2 with DRAQ7 to exclude dead cells.

Ekert et al., PLoS One 2014

http://dx.doi.org/10.1371/journal.pone.0092248

Surface expression assay for EGFR & cMET comparing 2D and 3D cultured NSCLC: PE-labeled anti-HGF/cMET and anti-EGFR antibodies, PE quantification beads and DRAQ7 to exclude dead cells.

DRAQ5 - for cell cycle analysis

Canham et al., PloS One 2014

http://dx.doi.org/10.1371/journal.pone.0115379

Cell cycle analysis assay in pre-neoplastic cervical disease: PE-labeled TRA-1-60 cell surface stem cell marker and DRAQ5 were combined to analyse cell cycle dynamics in cancer stem cell-like populations relative to neoplasia grading.

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