See:
Mathis et al. doi:10.1038/leu.2013.178
&
Geis et al. DOI: 10.1111/boc.201400079
.. for examples of multi-colour protocols using CYTRAK Orange to positively select intact cells from debris or necrotic/apoptotic cells.
This adds to the well-tested option of the related far-red DRAQ5 gating methodology.
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