Tuesday, 7 October 2014

CyGEL sorts suspension cell imaging!

A new publication by Sinkora and Sinkorova (Journal of Immunology, 2014. doi:10.4049/jimmunol.1401152) describes the use of CyGEL to capture immuno-phenotypically sorted B lymphocyte progenitors onto microscope slides.

This allows a detailed examination of the co-localisation of different markers on single cell events in a gentle procedure, needing only a chilled slide, chambered coverglass or microplate as the receiving vessel for the different populations that you want to sort.

After imaging, you can non-destructively recover the cells to analyse by other methods such as mass spectrometry or PCR.

This paper provides new insight into the development pathway and site of B cell lymphogenesis in swine.

#CyGEL

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