BioStatus is delighted to once more support the Autophagy UK Network's annual meeting at the University of Warwick
Here are some dedicated resources for cell biology assays in the study of autophagy, mitophagy, ..
First, a few numbers: you can use BioStatus products with confidence, being cited in more than 12,000 peer-reviewed articles. DRAQ5 and DRAQ7 have been cited in more than 1200 articles mentioning autophagy.
BioStatus's patented products are all UK inventions, manufactured in the UK, available direct for next-day delivery but also through carefully selected distribution partners.
Here are quick Google Scholar searches to help you..
Recent articles with autophagy in the title and using DRAQ5
Recent articles with autophagy in the title and using DRAQ7
DRAQ5 - the far-red, live-cell permeant DNA dye - enables multi-colour flow cytometry, fluorescence microscopy and high content imaging. At its simplest it's a counterstain for nucleated object gating or identification while at its most sophisticated it's a live-cell reporter of cell cycle. If you need cryo-EM then it is the perfect fiduciary marker that can bridge living tissue to ultrastructure (see blog).
DRAQ7 - the far-red, viability dye - allows you to add a viability dye into a flow cytometry panel where there doesn't seem to be room for one (see CYTO 2018 poster) or just enables smarter single tube apoptosis assays like AnnexinV / TMRM / DRAQ7 (see blog). It's become a reagent of choice in time-lapse imaging of cell death / survival in 2D and 3D cultures, being non-toxic and non-interfering (see BLSW 2015 poster and as used in the lab of the opening keynote speaker Prof Sylvie Urbé [Liang, et al. 2015]).
What else can BioStatus offer your research?
Single cell genomics & transcriptomics..
Both DRAQ5 & DRAQ7 feature strongly in single-cell and single-nuclei sorting protocols to aid delivery of quality templates for amplification and sequencing (see blog).
Hypoxia & autophagy..
HypoxiTRAK enables the time-lapse reporting of the accumulated hypoxic experience of individual cells in both 2D and 3D cultures, in hypoxia chambers and by imaging or flow cytometry. HypoxiTRAK's signal is consistent with Hif-1a expression. HypoxiTRAK requires no antibody detection nor does it futile redox cycle unlike the nitroimadazole based chemistries. (see blog).
High resolution imaging..
If you need to immobilize cells, model organisms or microtissues to image these at high resolution for colocalizations, FRAP or organelle detail then CyGEL may provide the answer. This thermo-reversible hydrogel is compatible with live cells and tissues and has ideal optical properties - clear, RI-matched to biological samples, negligible fluorescence.
Live cell tracking..
DRAQ9 - a novel non-toxic, far-red cell probe to follow cell migration in time-lapse studies (see MMC 2019 poster)
Any questions? Email us: enquiry@biostatus.com or visit www.biostatus.com
