Biotek Cytation™5 + DRAQ7™ for real-time cell death

A new application note authored by Brad Larson, Principal Scientist at Biotek Instruments, Inc. showcases their integrated BioSpa platform, which includes the Cytation™5, and the combination of three fluorescent probes to unpick and quantify mechanisms of cell death. 

The methodologies described should see wide applicability for the investigation of unwanted drug toxicity, cancer chemotherapeutic screening, personalised medicine, cell-based therapy and immunotherapy.  Far-red DRAQ7™ was selected as the reporter of cells with compromised membranes - signifying necrosis, mechanically-induced cell damage or a later feature of apoptosis.  The three reagents used were all chosen for their compatibility with real-time/time-lapse imaging

Automated, Multi-Parameter, Kinetic Methods to Quantify Cell Death - Application Note
Brad Larson, Principal Scientist, BioTek Instruments, Inc., Winooski, VT USA. March, 2018

An integrated, automated cell incubation and imaging workflow was used to demonstrate a multi-parametric and kinetic methodology to assess cell death: the BioSpa Live Cell Imaging System consists of the Cytation™5 Cell Imaging Multi-Mode Reader and BioSpa 8 Automated Incubator (Biotek Instruments, Inc.).

In the test system, fibrosarcoma HT1080 cells were treated with a 7-point, 4-fold dilution series of Camptothecin in the presence of TMRE (Abcam), pSIVA-IANDB (Abcam), and DRAQ7™ to detect mitochondrial membrane potential, externally-exposed phosphatidylserine and compromised cell membranes / necrosis respectively.  Wells were imaged at 2-hourly intervals for 48 hours for the three chromophore signals and for brightfield to assess cell numbers.

WHERE TO BUY