Wednesday, 19 December 2012

DRAQ7 Improves SP Analysis


DRAQ7™ permits a new kinetic understanding of SP analysis.  

  • Membrane-impermeant DRAQ7™ allows removal of interfering Hoechst signals coming from dead and damaged cells. 
  • DRAQ7™ is non-toxic, does not passively enter intact cells during the extended exposure times required and also allows sorting of the viable SP sub-populations. 
  • DRAQ7™ has no UV-excitation, avoiding PI's red emission overlap. 
  • Far-red DRAQ7™ permits multi-colour phenotypic SP analysis.


REFERENCE:

Smith, P.J. et al. Kinetic analysis of intracellular Hoechst 33342-DNA interactions by flow cytometry: Misinterpretation of side population status?
Cytometry Part A. Early View - published online November 7, 2012 DOI: 10.1002/cyto.a.22224
Access this article here

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